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Figure 2 | Molecular Cancer

Figure 2

From: LEDGF/p75 has increased expression in blasts from chemotherapy-resistant human acute myelogenic leukemia patients and protects leukemia cells from apoptosis in vitro

Figure 2

The expression and characterization of LEDGF/p75 splice variants in NB4 cells. A) The splicing pattern of LEDGF/p75 mRNA in NB4 cells as revealed by RT-PCR. The primers (p75-1; from exons 1 and 9) used for lane 1 (left) and (p75-2; exons 3 and 8) used for lane 2 (right) were from the common region of LEDGF/p75 and p52. The identity of the splice variants marked in lane 2 was verified by DNA sequencing. B) Schematic representation of the p52 splice variants. The identical N-terminal domain of LEDGF/p75 and p52 is indicated as exon 1 to exon 9. The p52 variant contains an alternative exon 9b with a poly (A) tail at its C-terminal end, whereas C) p52b differs from p52 by lacking 55 bases in the alternative exon 9b. The low expression p52bΔE6 variant lacks exon 6 at its N-terminal region and has a frameshift that introduces several stop codons, whereas p52Δ1 and p52bΔ1 lack exons 6, 7 and parts of 5 and 8. // indicates omitted exons in the figure.

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Molecular Cancer

ISSN: 1476-4598