O 6 -methylguanine-DNA methyltransferase is downregulated in transformed astrocyte cells: implications for anti-glioma therapies

Table 1 Summary of soft agar colony formation assay and xenograft propagation experiment: NHA and NIH3T3 cells infected with retroviral vectors expressing hTERT (T), SV40ER (S), H-RasV12 (R), myrAKT (A), and/or MGMT as well as parental NHA cells were subjected to the soft-agar colony formation and xenograft propagation assays.

^aNHA (2 × 10⁴) and NIH3T3 cells (1 × 10⁴) were plated in soft-agar (0.36% top agar containing 5% fetal calf serum in 60-mm dishes) and incubated for 21 days and 14 days, respectively. Colonies were stained with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, and the numbers of stained colonies were counted. Results represent mean ± SD from two independent triplicate experiments.
^b3 × 10⁶ cells were injected s.c. into nude mice. n, number of animals treated. ND, not done.

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