Effects of AR siRNA-knockdown on gene expression. C4-2B cells were treated with AR siRNA (white bars) or a non-specific siRNA (black bars), followed by administration of either DHT (10 nM) or Ethanol vehicle for 16 hours. Expression levels of the indicated genes were analyzed in triplicate by RT-qPCR and corrected for 18S rRNA levels. Values measured with the non-specific siRNA and ethanol were defined as 1. Results (Mean ± SD) are representative of three independent experiments.