Quantitative real-time PCR validation of microarray results. Validation of selected 8 SAM-identified genes by quantitative real-time PCR. BCL2L10, HAP1 and GAS1 were up-regulated in TGCT cells and down-regulated in HCT116 cells following cisplatin exposure. DICER1, NEO1, IL6ST, NFRKB and SSBP2 were up-regulated in HCT116 cells and down-regulated in TGCT cells. The bars indicate the mean +/- S.E.M.