Figure 3
From: Sodium Ascorbate induces apoptosis in neuroblastoma cell lines by interfering with iron uptake
Reduction of intracellular iron levels by sodium ascorbate. 30 × 106 of either HTLA-230 or SH-SY5Y were treated with the indicated doses of sodium ascorbate for 24 hours and iron levels were measured in cell lysates. After terminating the incubation, cells were collected by scraping and washed three times with PBS and then lysed in specific buffer. Iron levels were analyzed with a Cobas Integra 800 system as described under "Material and Method". Values indicated are the mean ± S.D. of three separate experiments.