Figure 4From: Selective repression of retinoic acid target genes by RIP140 during induced tumor cell differentiation of pluripotent human embryonal carcinoma cellsGenes differentially expressed with RA in RIP140 siRNA treated NT2/D1 cells. Five of top six genes in which RA-regulation was altered in RIP140 siRNA cells (Table 1) were confirmed by RT-PCR analysis. Samples used were the same as those used in the microarray analysis (Figure 2C). Cells were mock transfected (no siRNA) or transfected with RIP140 siRNA#1 or RIP140 siRNA#2 prior to treatment with vehicle or 10 μM RA for 24 hours. In indicated samples (-RT) reverse transcriptase was omitted to control for genomic DNA contamination.Back to article page