Figure 5
From: Differential effects of hypoxia on etoposide-induced apoptosis according to the cancer cell lines
Effect of hypoxia on the etoposide-induced p53 activation. A549, MCF-7 or HepG2 cells were incubated under normoxic or hypoxic conditions with or without etoposide (50 μM) for 1 or 16 hours. A, after the incubation, nuclear extracts were performed from three independent experiments and hybridized in the ELISA well containing specific DNA probes (TransAM assay). Detection was performed using an anti-p53 antibody. Results are expressed in absorbance (means ± 1 SD, n = 3). B, Cells were cotransfected with the reporter plasmid pG13-Luc encoding the firefly luciferase and the pCMVβ normalization plasmid before being incubated 16 hours under normoxia (N) or hypoxia (H) in the presence or absence of etoposide (e, 50 μM). Results are expressed as means of the ratio between firefly luciferase activity and the β-galactosidase activity ± 1 SD (n = 3).