Figure 9
From: Differential effects of hypoxia on etoposide-induced apoptosis according to the cancer cell lines
Effect of hypoxia and/or etoposide on p21 (A), Bak, Bax and mdm2 (B) protein levels. A549, MCF-7 or HepG2 cells were incubated under normoxic (N) or hypoxic (H) conditions with or without etoposide (e, 50 μM) for 16 hours. p21, Bak, Bax and mdm2 were detected in total cell extracts by western blotting, using specific antibodies. a-tubulin was used to assess the total amount of proteins loaded on the gel.