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Table 2 Cell cycle analysis of wild type and PP2Cα siRNA-expressing MCF7 cells upon treatment with radiotherapy.

From: Role of PP2Cα in cell growth, in radio- and chemosensitivity, and in tumorigenicity

  

MCF7-wt

MCF7-si

Time (h)

RT Dose (Gy)

G0/G1

S

G2/M

G0/G1

S

G2/M

24

0

61.3 ± 0.5

29.5 ± 1.1

9.2 ± 1.5

55.6 ± 0.5 *

30.3 ± 0.4

14.2 ± 0.7 *

 

2

68.3 ± 0.2

21.8 ± 0.4

9.9 ± 0.4

61.4 ± 1.3 *

25.4 ± 1.1 *

13.2 ± 0.5 *

 

4

71.6 ± 0.2

16.7 ± 0.2

11.7 ± 0.3

67.4 ± 6.2 *

25.0 ± 5.7 *

7.5 ± 0.9 *

 

6

70.9 ± 0.6

14.0 ± 0.4

15.1 ± 0.7

77.5 ± 0.9 *

9.2 ± 1.5 *

13.3 ± 0.6 *

 

12

66.8 ± 0.5

12.6 ± 0.3

20.6 ± 0.5

54.8 ± 0.9 *

3.4 ± 0.3 *

41.8 ± 0.8 *

48

0

61.5 ± 0.6

35.4 ± 1.4

12.7 ± 1.3

61.2 ± 1.8 *

31.0 ± 1.2 *

12.0 ± 0.4

 

2

62.9 ± 8.0

28.1 ± 5.5

9.0 ± 2.5

69.2 ± 0.4

21.4 ± 0.2

9.4 ± 0.4

 

4

75.9 ± 6.7

15.8 ± 8.0

8.2 ± 1.3

77.9 ± 0.2

11.3 ± 0.4

10.8 ± 0.6 *

 

6

79.2 ± 1.0

7.4 ± 2.8

13.7 ± 3.4

74.4 ± 0.9 *

5.8 ± 0.4

19.8 ± 1.3 *

 

12

55.3 ± 1.1

7.2 ± 3.7

37.3 ± 3.1

48.8 ± 0.4 *

2.6 ± 0.4

48.6 ± 0.4 *

72

0

51.1 ± 1.3

42.4 ± 2.0

6.4 ± 1.2

54.0 ± 2.6

33.6 ± 0.2 *

12.6 ± 2.9 *

 

2

61.1 ± 0.3

26.6 ± 1.1

12.3 ± 1.0

61.9 ± 0.6

24.7 ± 0.1 *

13.4 ± 0.6

 

4

65.1 ± 1.7

21.2 ± 1.1

13.7 ± 1.3

66.2 ± 0.9

18.5 ± 0.9 *

15.3 ± 1.5

 

6

66.4 ± 0.6

14.7 ± 0.8

18.9 ± 0.9

68.4 ± 0.6 *

13.8 ± 0.4

17.9 ± 0.1

 

12

51.7 ± 0.7

8.1 ± 0.6

40.3 ± 0.2

54.9 ± 0.3 *

6.4 ± 0.8

38.7 ± 0.2 *

  1. Multicycle algorithm-based quantification of the amounts of MCF7-wt and MCF7-si cells in the G0/G1, the S and the G2/M phase of the cell cycle at 24, 48 and 72 h after treatment with the indicated doses of radiotherapy. Values represent average ± SD (n = 3). * Indicates p < 0.05 vs. MCF-wt.