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Table 2 Cell cycle analysis of wild type and PP2Cα siRNA-expressing MCF7 cells upon treatment with radiotherapy.

From: Role of PP2Cα in cell growth, in radio- and chemosensitivity, and in tumorigenicity

   MCF7-wt MCF7-si
Time (h) RT Dose (Gy) G0/G1 S G2/M G0/G1 S G2/M
24 0 61.3 ± 0.5 29.5 ± 1.1 9.2 ± 1.5 55.6 ± 0.5 * 30.3 ± 0.4 14.2 ± 0.7 *
  2 68.3 ± 0.2 21.8 ± 0.4 9.9 ± 0.4 61.4 ± 1.3 * 25.4 ± 1.1 * 13.2 ± 0.5 *
  4 71.6 ± 0.2 16.7 ± 0.2 11.7 ± 0.3 67.4 ± 6.2 * 25.0 ± 5.7 * 7.5 ± 0.9 *
  6 70.9 ± 0.6 14.0 ± 0.4 15.1 ± 0.7 77.5 ± 0.9 * 9.2 ± 1.5 * 13.3 ± 0.6 *
  12 66.8 ± 0.5 12.6 ± 0.3 20.6 ± 0.5 54.8 ± 0.9 * 3.4 ± 0.3 * 41.8 ± 0.8 *
48 0 61.5 ± 0.6 35.4 ± 1.4 12.7 ± 1.3 61.2 ± 1.8 * 31.0 ± 1.2 * 12.0 ± 0.4
  2 62.9 ± 8.0 28.1 ± 5.5 9.0 ± 2.5 69.2 ± 0.4 21.4 ± 0.2 9.4 ± 0.4
  4 75.9 ± 6.7 15.8 ± 8.0 8.2 ± 1.3 77.9 ± 0.2 11.3 ± 0.4 10.8 ± 0.6 *
  6 79.2 ± 1.0 7.4 ± 2.8 13.7 ± 3.4 74.4 ± 0.9 * 5.8 ± 0.4 19.8 ± 1.3 *
  12 55.3 ± 1.1 7.2 ± 3.7 37.3 ± 3.1 48.8 ± 0.4 * 2.6 ± 0.4 48.6 ± 0.4 *
72 0 51.1 ± 1.3 42.4 ± 2.0 6.4 ± 1.2 54.0 ± 2.6 33.6 ± 0.2 * 12.6 ± 2.9 *
  2 61.1 ± 0.3 26.6 ± 1.1 12.3 ± 1.0 61.9 ± 0.6 24.7 ± 0.1 * 13.4 ± 0.6
  4 65.1 ± 1.7 21.2 ± 1.1 13.7 ± 1.3 66.2 ± 0.9 18.5 ± 0.9 * 15.3 ± 1.5
  6 66.4 ± 0.6 14.7 ± 0.8 18.9 ± 0.9 68.4 ± 0.6 * 13.8 ± 0.4 17.9 ± 0.1
  12 51.7 ± 0.7 8.1 ± 0.6 40.3 ± 0.2 54.9 ± 0.3 * 6.4 ± 0.8 38.7 ± 0.2 *
  1. Multicycle algorithm-based quantification of the amounts of MCF7-wt and MCF7-si cells in the G0/G1, the S and the G2/M phase of the cell cycle at 24, 48 and 72 h after treatment with the indicated doses of radiotherapy. Values represent average ± SD (n = 3). * Indicates p < 0.05 vs. MCF-wt.