Figure 4

Western blot analysis of effect of nilotinib on the tyrosine kinase activity of Bcr/Abl in vivo. S-5 and S-9 are lymphoma lysates prepared from two mice transplanted with 8093 cells in the nilotinib-treated group 23 hours and 2 hours after the last nilotinib treatment respectively. Lanes A-5, A-21 and 8093 contain lysates prepared from lymphoma cell lines A-5, A-21 and 8093. 8093 is the parental cell line and A-5, A-21 lymphoma cells were isolated from two mice in the nilotinib treatment group and cultured ex vivo. All leukemia cell lysates shown here are from mice, which developed lymphoma during Nilotinib treatment. Membranes were reacted with antibodies indicated below each panel. Arrows indicate the positions of P190 Bcr/Abl, P160 Bcr (endogenous mouse) and phosphorylated and non-phosphorylated Crkl.