Effect of TGF-β2 on 3H-thymidine incorporation in human pancreatic cancer cells. Treatment of CAPAN-2 (top panel) and HS766T cells (bottom panel) with TGF-β2 significantly inhibited incorporation of 3H-thymidine in a concentration-dependent manner. A significant time-dependent effect was seen in Capan-2, but not Hs766T cells. Two-way ANOVA: Capan-2, Concentration effect F(7,32) = 181; Time effect F(1,32) = 104; Interaction F(7,32) = 18.2, all P < 0.0001; Hs766T: Concentration effect F(7,32) = 26.1, P < 0.0001; Time effect F(1,32) = 1.72, P = 0.199; Interaction F(7,32) = 4.96, P = 0.0007. Data are expressed in counts per minute (CPM). Bars represent means ± SEM.