Figure 1

PSAT1 mRNA expression level and clinical correlation between PSAT1 mRNA expression level and tumoral regression. Box plot representation of PSAT1 mRNA levels determined by Affymetrix technology (A) and by semiquantitative PCR (B). NC: normal colon (17 samples). TC: tumor colon (23 samples). HM: hepatic metastasis (22 samples). The box for each gene represents the interquartile range (25–75th percentile) and the line within this box is the median value. Bottom and top bars of the whisker indicate the 10th and 90th percentiles, respectively. Outlier values are indicated (closed squares). The clinical data for the 29 patients included in this study are detailed in Table 1. C, PSAT1 mRNA levels determined by semiquantitative PCR in various cell lines. NC: normal colon (17 samples). TC: tumor colon (23 samples). D, PSAT1 mRNA expression in hepatic metastasis before (NT: not treated) and after (T: treated) chemotherapy determined by semi-quantitative RT-PCR. Patients' response to treatment (tumor regression) and PSAT1 expression after therapy are negatively correlated (Pearson's test, p = -0.76).