Figure 1
From: DNMT3b overexpression contributes to a hypermethylator phenotype in human breast cancer cell lines
Expression analysis of methylation-sensitive genes in human breast cancer cell lines. (A) Representative agarose gels of RT-PCR products from CST6, LCN2, SCNN1A, CDH1, CEACAM6, ESR1, and β-actin. The source of cDNA template is identified for each lane. Normal breast epithelial MCF12A cells represent the positive control cell line. (B) Quantitative real-time PCR results for CST6, LCN2, SCNN1A, CDH1, CEACAM6, and ESR1. Black bars correspond to hypermethylator cell lines, cross-hatched bars correspond to low-frequency methylator cell lines, and the white bar (far right) corresponds to MCF12A cells (index control cell line). The expression level of each gene is depicted relative to that of MCF12A cells. Error bars represent S.E.M. Instances of no detectable level of quantitative real-time PCR expression are indicated by an asterisk (*). (C) Unsupervised cluster analysis for 48 genes that are expressed at a detectable level in MCF12A cells. The 12 breast cancer cell lines group into two distinct clusters, designated cluster I (corresponding to hypermethylator cell lines) and cluster II (corresponding to low-frequency methylator cell lines).