Figure 3

Cisplatin-induced monoubiquitylation of FANCD2 is not affected by phenylbutyrate in UM-SCC-1, -6 and -25 cell lines. Cells were treated with 2 mM phenylbutyrate (PB) for 48 hours before adding 5 μM cisplatin and incubated for an additional 24 hours. *represents the slower migrating monoubiquitylated form of FANCD2.