Figure 1

Proliferation of mouse lymphocytes and lymphoma cells in vitro in the presence and absence of L-744,832. Splenocytes were harvested from either a BCR^HEL transgenic mouse (panels a-e) or a moribund Eμ-Myc/BCR^HEL/HEL transgenic mouse (panels f-j). The splenocytes were depleted of T cells and labeled with CFSE. Cells were cultured in RPMI (panels a, f-j) or RPMI with 2 μg/ml anti-IgM and 1 μg/ml anti-CD40 (panels b-e) and 0 – 40 μM L-744,832 were included in the culture media, as indicated. After three days, cellular proliferation was measured by flow cytometry. Cells that have proliferated measure less than 10³ fluorescence units. For comparison, panels c, d, and e show proliferation in the absence of L-744,832 as a black line.