Figure 6

Real-time RT PCR study of 22 genes differentially expressed between 1p19q codeleted gliomas and EGFR amplified gliomas. Real-time RT-PCR study of 11 genes overexpressed in gliomas with EGFR amplification (top) and 11 genes overexpressed in gliomas with 1p19q codeletion (bottom) was performed in an independent data set of 16 gliomas (8 gliomas with EGFR amplification (triangles), 8 gliomas with 1p19q codeletion (circles)). Each dot represents the relative expression (log2 transformed) of a given gene in one glioma compared with normal brain (median expression in the 3 normal brain samples). Dots above the upper dashed line are upregulated with a fold change larger than 2 in comparison to normal brain; dots below the lower dashed line are downregulated in comparison to normal brain with a fold change larger than 2. For example, NOG, BMP2 and ATOH8 were overexpressed in all 8 gliomas with 1p19q codeletion (circles) in comparison to all 8 gliomas with EGFR amplification (triangles) and in comparison to normal brain. CHI3L1, PLAT, IQGAP1, IGFFBP2 and GBP1 were overexpressed in all gliomas with EGFR amplification (triangles) in comparison to gliomas with 1p19q codeletion (circles) and in comparison to normal brain. Except for NCAM1, all 22 genes were differentially expressed (p < 0.05).