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Figure 5 | Molecular Cancer

Figure 5

From: Altered splicing of CEACAM1 in breast cancer: Identification of regulatory sequences that control splicing of CEACAM1 into long or short cytoplasmic domain isoforms

Figure 5

Identification of CEACAM1 regulatory sequences required for the splicing of exon 7. (A) Schematic representation of CEACAM1 minigene or chimeras cloned into pcDNA 3.1/Myc-His(-) C. Open or shaded boxes are exons and lines represent introns. The name of each minigene is indicated on right. The size of middle exon in all minigenes is identical to exon 7 of CEACAM1. In CAM 6-βg-8 the entire middle exon is replaced by 53 bp of β-globin exon 2. In minigenes CAM 6-E7.1βg-8 and CAM 6-E7.2βg-8, the first and the last 20 bp (E7.1 and E7.2 regions) of CEACAM1 exon 7 are replaced by β-globin exon 2 sequences. The forward (T7) and reverse priming sites (BGH) are indicated. (B and C) RT-PCR analysis of RNA derived from MDA-MB468 and ZR75 cells transiently transfected with indicated minigenes or empty vector. -RT represents omission of reverse transcriptase during cDNA synthesis. The data shown is representative of three independent experiments. Bar diagrams represent the mean ± SD of at least three independent experiments. *P < 0.001 and **P < 0.01 versus CAM 6-7-8.

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Molecular Cancer

ISSN: 1476-4598