Figure 5

A – Expression of VEGF, TNF-α and IFN-α was detected in the tumors using western immunoblot analysis. Expression of actin was used to monitor protein loading. Higher protein expression was detected in cellular targeted long DLI PDT at 24 h compared to short DLI PDT at 24 h. TNF-α is secreted predominantly by the macrophages and its downregulaton at short DLI 30 days could suggest minimal macrophage recruitment due to extensive vascular damage. At 30 days short and long DLI PDT comparable levels of IFN-α and VEGF was noticed. B – Ratio of VEGF, IFN-α and TNF-α density was plotted against actin. Compared to control, expression of proteins were statistically significant (p < 0.05) for all the groups except IFN-α at long DLI 30 days PDT group. Each group represents the mean (bars, SE) of 8 tumors.