Figure 1

Identification of chromosome 5p genomic alterations in cervical cancer. A-B. Copy number alterations in log2 ratio of chromosome 5 identified by 250 K Nsp I SNP array. Each vertical column represents a sample with genomic regions representing from pter (top) to qter (bottom). Prefix "T" indicates primary tumor; "CL" indicates cell line. The blue-red scale bar (-1 to +1) at the bottom represents the copy number changes relative to mean across the samples. The intensities of blue and red indicate relative decrease and increase in copy numbers, respectively. G-banded ideogram of chromosome 5 is shown on the extreme right in each panel. A. Identification of 5p gain as the most frequent genomic alteration in CC. All tumors that exhibited 5p gain are shown. Inferred copy number view of HeLa cell line showing 5p gain from normal (2N) (red line) is shown on right. B. 5q deletions in CC. All tumors that exhibited 5q deletions arranged from largest to smallest deletion are shown. Inferred copy number view of T-1875 showing 5q deletion from normal (2N) (red line) is shown on right. C-F. Fluorescence in situ hybridization (FISH) identification of 5p gains in invasive cancer (C-E) and high-grade cervical intra-epithelial lesion (HSIL) (F). Green signals represent 5p15.2 probe and Red signals represent probe mapping to 5q31 region used as control. Panels C-D represent invasive CC cases with increased copies of 5p (green) compared to 5q (red). Note the concordance of SNP copy number changes (panel A) and increased copies of 5p by FISH (panels C-E) for tumors T-207, T-218, and T-1981, respectively. Panel F showing 4 copies of chromosome 5p and 5q (tetrasomy 5) on a pap smear from HSIL.