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Figure 6 | Molecular Cancer

Figure 6

From: The parafibromin tumor suppressor protein interacts with actin-binding proteins actinin-2 and actinin-3

Figure 6

Location of endogenous parafibromin in HEK293 cells transfected with actinin. HEK293 cells transfected with plasmid expressing FLAG-actinin-2 were analyzed by immunofluorescence with primary antibodies that recognize parafibromin (GRAPE) or the FLAG-tag (actinin-2), and secondary antibody conjugated to Texas Red (B, to detect endogenous parafibromin) or fluorescein isothiocyanate (C, to detect transfected actinin-2), followed by DAPI staining (A). Note that in untransfected cells parafibromin is nuclear but in the 3 cells over-expressing FLAG-actinin-2, parafibromin is in the cytoplasm. Merged Texas Red (parafibromin) and fluorescein isothiocyanate (actinin-2) signal is shown in panel D. Magnification, 60×.

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Molecular Cancer

ISSN: 1476-4598