Figure 6

Semi-quantitative RT-PCR post-validation of microarray results. A. Gene expression changes for seven genes (CDKN1A, GADD45, TXNIP, MYC, GEM, AREG, EREG) were measured at selected time points after CPT treatment by semi-quantitative RT-PCR and normalized to β-actin expression of time- and dose- matched controls. The RT-PCR reactions were analyzed with the DNA1000 kit of the Agilent 2100 Bioanalyzer to give a gel-like image. B. At each selected time point after CPT treatment, expression fold change values obtained with semi-quantitative RT-PCR and microarrays were compared.