Figure 1From: Differential effects of class I isoform histone deacetylase depletion and enzymatic inhibition by belinostat or valproic acid in HeLa cellsEffects of HDAC depletion by siRNA in HeLa cells. A) Down-regulation of HDAC1, 2 or 3 protein after treatment with HDAC1, 2 or 3-targeted siRNA respectively, alone or in combination with other siRNAs at 72 hours post-transfection. Top-row is the appropriate antibody (HDAC1, 2 or 3), while the bottom-row is loading control actin; B) viability as measured by ATP present in culture. For each HDAC knockdown condition, viability was consistently reduced as compared to scrambled siRNA control, in independent measurements (p < 0.01, n = 24); C) The activity of effector caspases 3 and 7 corresponding to apoptotic progression upon HDAC down-regulation or belinostat treatment at increasing concentrations for 24 hours for comparison; D) flow cytometric analysis of DNA content as a function of cell number in control or HDAC combination KD at 48 hours post-transfection, or belinostat treatment at 5.0 μM for 24 hours.Back to article page