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Figure 2 | Molecular Cancer

Figure 2

From: Human ESCs predisposition to karyotypic instability: Is a matter of culture adaptation or differential vulnerability among hESC lines due to inherent properties?

Figure 2

Molecular cytogenetic analysis of SHEF-3 hESC line. SHEF-3 hESC line was genetically stable for 51 passages in feeders. Upon being transferred to feeder-free conditions and passaged using enzymatic methods, it quickly gained a trisomy of chromosome 14 as early as in p10. Opposite to the HS181 line, the degree of mosaicism for the extra chromosome 14 did not increase overtime in feeder-free conditions and remained between 23%–36% over the culture (see Table 1). In conventional karyotyping analysis (A), the blue arrows indicate chromosomal gains. B,C: SKY and CGH analyses showing the absence of further structural or numerical abnormalities.

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Molecular Cancer

ISSN: 1476-4598