Bone marrow ectopic expression of a non-coding RNA in childhood T-cell acute lymphoblastic leukemia with a novel t(2;11)(q11.2;p15.1) translocation

Guastadisegni, Maria Corsignano; Lonoce, Angelo; Impera, Luciana; Albano, Francesco; D'Addabbo, Pietro; Caruso, Sebastiano; Vasta, Isabella; Panagopoulos, Ioannis; Leszl, Anna; Basso, Giuseppe; Rocchi, Mariano; Storlazzi, Clelia Tiziana

doi:10.1186/1476-4598-7-80

Molecular Cancer

Table 1 Primers for PCR and sequencing.

From: Bone marrow ectopic expression of a non-coding RNA in childhood T-cell acute lymphoblastic leukemia with a novel t(2;11)(q11.2;p15.1) translocation

Designation^a	Sequence (5'->3')	Position^b	Gene
chr2-97-1F	AGCCTGTGGTGTGTGTATGAAC	chr2:97,380,441–97,380,462	-
chr2-97-1R	TGAGTGTAGGTGACTGGTGAGG	chr2: 97,391,465–97,391,486	-
hpsgtf-R	TGACACCTTCCGCTAGTTCC	chr11:18,300,606–18,300,625	-
Hps5-F	CCTCCCTGCTTCTTTTTCCA	chr11:18,299,339–18,299,358	HPS5
HPS5ex20-3216F^c	TGCAGGTCTTGTGGTTTCTG	chr11:18,263,475	HPS5
HPS5ex21-3255R^c	TCTTCTCTCCAGCTCCAAACA	chr11:18,261,979–18,261,999	HPS
HPS5ex1-19F^c	TTCACGTTCCGCTCTTAGTG	chr11:18,300,260–18,300,279	HPS5
HPS5ex1-96R^c	CACATCCAGGGCAGTACCTC	chr11:18,300,183–18,300,202	HPS5
HPS5ex1-181F	TGGTTACTGGGTCTCCTCTCA	chr11:18,300,107–18,300,127	HPS5
HPS5-intr1-F	TGTGTTTTGTTCATCCCTGTG	chr11:18,300,006–18,300,026	HPS5
DA926692 ex1F^c	TCAGTCCCAGTCAGGACACA	chr2:97,384,972–97,384,991	DA926692
DA926692 ex1R^c	AGAGCCAGAGCAGCAGGAG	chr2:97,384,918–97,384,936	DA926692
DA926692 ex2aF	CCATCAAGGGAAGCAGATGT	chr2:97,384,457–97,384,476	DA926692
DA926692 ex2aR	GAGGCACCAGGAGAAGCAT	chr2:97,384,418–97,384,436	DA926692
DA926692ex2anewF^c	CCCACAGTGTTACAAGTCATAACATA	chr2:97,384,479–97,384,504	DA926692
DA926692ex2anewR^c	AGGAAGCTTCATGGCTCCTT	chr2:97,384,334–97,384,353	DA926692
Beta-act F^c	CTGGAACGGTGAAGGTGACA	chr7:5,533,739–5,533,758	ACTB
Beta-act R^c	AAGGGACTTCCTGTAACAACGCA	chr7:5,533,619–5,533,641	ACTB
CD110063-R	CACCAGGAGGCAGACGAG	chr2:97,391,951–97,391,968	CD110063
ACTB-F	GGCATCGTGATGGACTCCG	chr7:5,534,773–5,534,789	ACTB
ACTB-R	GCTGGAAGGTGGACAGCGA	chr7:5,533,972–5,533,990	ACTB
GTF2H1ex9-1201F	AGCAGTCAAAAGGGCGAAAT	chr11:18,326,030–18,326,043/18,330,004–18,330,010	GTF2H1
GTF2H1ex10-1272R	TTCTTGAGGTTTAGTGCAATCG	chr11:18,330,062–18,330,083	GTF2H1
ANKex2-351F	CCAACCGGAAATGGTACATC	chr2:97,147,558–97,147,577	ANKRD36
ANKex2-451R	AGAGTTGCACAAGCCTCCTG	chr2:97,147,823–97,147,842	ANKRD36
FAHD2Bex5-829F	TGTAGCAGATCCACACAACTTAAA	chr2:97,113,730–97,113,742/97,115,163–97,115,171	FAHD2B
FAHD2Bex6-864R	GACGACTTCCCCATTCACTC	chr2:97,113,695–97,113,714	FAHD2B
KIAA1641ex2-214F	AGAAAATGGGATGCAGGATT	chr2:97,492,508–97,492,527	KIAA1641
KIAA1641ex2-245R	GAAATTGACTTCTCATCTGGTCTAA	chr2:97,490,960–97,490,957/chr2:97,492,476–97,492,496	KIAA1641
ZAP70ex11-1678F	AGCTACTACACTGCCCGCTCA	chr2:97,720,549–97,720,560/97,720,651–97,720,660	ZAP70
ZAP70ex11-1749R	CTGCGGCTGGAGAACTTG	chr2:97,720,709–97,720,726	ZAP70

^a F denotes forward; R denotes reverse;
^b Position, at nucleotide level, according to the UCSC database, using the BLAT tool http://genome.ucsc.edu/cgi-bin/hgBlat;
^cPrimer used in Real-Time PCR analysis.

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ISSN: 1476-4598

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