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Figure 8 | Molecular Cancer

Figure 8

From: Membrane androgen receptor activation triggers down-regulation of PI-3K/Akt/NF-kappaB activity and induces apoptotic responses via Bad, FasL and caspase-3 in DU145 prostate cancer cells

Figure 8

Testosterone-BSA induces Caspase-3 activation. A) Caspase-3 activity was measured at 405 nm in lysates derived from cells exposed to 10-7 M testosterone-BSA for the indicated time points and then incubated with the caspase-3 substrate DEVD conjugated to the chromophor pNA as described in Methods and Materials. B) Cells were pre-treated or not with 10-7 M of cytochalasin B or Y-27632 (10 μM) for 1 h and then exposed or not to 10-7 M testosterone-BSA for 3 h. The relative caspase-3 activity is expressed as percentage with that of serum cultured cells taken as 100%. Data presented in bars are mean values ± SE of three independent experiments (*P < 0.05).

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Molecular Cancer

ISSN: 1476-4598