Expression of Akt and its downstream effectors following co-treatment. A, After 48 h of exposure to the indicated agents, cell lysates were subjected to western blotting using antibodies against phospho-Akt (Ser473), total Akt, phospho-caspase-9 (Ser136), caspase-9, XIAP or survivin. B and C, After exposure to the agents for the periods indicated, cell lysates were subjected to western blotting with anti-phospho-Akt (Ser473), anti-Akt (B), anti-phospho-mTOR (Ser2448), and anti-mTOR (C) antibodies. β-actin was used as a loading control. -, treated with DMSO. GEN, genistein.