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Figure 3 | Molecular Cancer

Figure 3

From: A combination of indole-3-carbinol and genistein synergistically induces apoptosis in human colon cancer HT-29 cells by inhibiting Akt phosphorylation and progression of autophagy

Figure 3

Expression of Akt and its downstream effectors following co-treatment. A, After 48 h of exposure to the indicated agents, cell lysates were subjected to western blotting using antibodies against phospho-Akt (Ser473), total Akt, phospho-caspase-9 (Ser136), caspase-9, XIAP or survivin. B and C, After exposure to the agents for the periods indicated, cell lysates were subjected to western blotting with anti-phospho-Akt (Ser473), anti-Akt (B), anti-phospho-mTOR (Ser2448), and anti-mTOR (C) antibodies. β-actin was used as a loading control. -, treated with DMSO. GEN, genistein.

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Molecular Cancer

ISSN: 1476-4598