Figure 1From: Expression and function of hypoxia inducible factor-1 alpha in human melanoma under non-hypoxic conditionsExpression of HIF-1α protein in human melanocytes and melanoma cells. Nuclear extracts (30 μg) from HEMn-LP (normal human melanocytes), SbCl2 (radial growth phase melanoma), WM1366 (vertical growth phase melanoma) and WM9 (metastatic melanoma) (A) and WM3211 (radial growth phase melanoma), WM3248 (vertical growth phase melanoma), WM239 (metastatic melanoma) (B) cell lines were analyzed by western blotting using a monoclonal anti-HIF-1α antibody (1 μg/ml). LaminB1 was used as a loading control. This blot is representative of at least 3 different experiments, all of which gave similar results.Back to article page