Figure 3
From: Dual effects of TGF-β on ERα-mediated estrogenic transcriptional activity in breast cancer
Smad4 is essential for the inhibitory effect of TGF-β in ERα-mediated transcriptional activation. (a) RNAi was performed in MCF-7 cells using Smad4 siRNA (see materials and methods). (b) MCF-7 cells were transfected with either siRNA targeting Smad4 or scrambled siRNA (as a control). Sixty hours after transfection, the cells were treated with (+) or without (-) E2 or TGF-β for 6 hrs. Total RNA was isolated and the mRNA levels of PS2 and C-MYC were quantitated by RT-PCR.