PCR detection of hedgehog signaling in ovarian cancer specimens. A. Real-time PCR analysis of GLI1 expression in ovarian cancer was performed as described in Materials and Methods. Gli1 transcript was shown here. B. PCR detection of SHH, PTCH1, GLI1, SMO, HIP1 and Su(Fu) transcripts in ovarian cancers. GAPDH is the endogenous control. Numbers listed indicate specimen number.