Membrane staining of mAR in colon tumors and colon cancer cells. Confocal laser scanning microscopic analysis of WCL2 (a-c) and MCL2 (d-f) colon tumor specimens and HCT116 cells (g-j) stained with testosterone-HSA-FITC, showing specific FITC related fluorescence at the cell membranes. Control staining with HSA-FITC showed no apparent membrane fluorescence. Visualization of nuclei was evident by DAPI or TO-PRO-3 staining. Magnification, ×100.