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Figure 3 | Molecular Cancer

Figure 3

From: Identification of iGb3 and iGb4 in melanoma B16F10-Nex2 cells and the iNKT cell-mediated antitumor effect of dendritic cells primed with iGb3

Figure 3

Stimulation of DN32D3 NKT cells by cytosolic and membrane fractions of murine melanoma B16F10-Nex2. BMDCs were pulsed with different concentrations of (A) Cytosolic and (B) Membrane fractions, both extracted from in vivo growing murine melanoma B16F10-Nex2. Primed BMDCs were co-cultured with NKT hybridoma cells as described in Material and Methods, and the IL-2 production was measured in the supernatants by ELISA. Ctr, DN32D3 cells stimulated with untreated BMDCs.

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