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Figure 8 | Molecular Cancer

Figure 8

From: Identification of iGb3 and iGb4 in melanoma B16F10-Nex2 cells and the iNKT cell-mediated antitumor effect of dendritic cells primed with iGb3

Figure 8

iGb3-stimulated DN32D3 NKT cells are cytotoxic to B16F10-Nex2 melanoma cells. A) BMDCs were pulsed with iGb3 for 24 h, co-cultured with DN32D3 NKT cells for 18 h, and IL-2 production was measured in the supernatant by ELISA. The results are representative of three independent experiments. B) BMDCs were primed with 20 μg/ml iGb3 for 24 h and co-cultured with NKT hybridoma cells for 4 h. B16F10-Nex2 cells were previously incubated with 5 μCi of [3H] thymidine for 24 h. Melanoma and NKT cells were co-cultured at the target/effector cell ratio indicated and the cytotoxic (lytic) effect was measured as described in Material and Methods.

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