Inhibition of genomic instability by restoring the correct CenpA protein level in RB-depleted cells. A) Detection of Mpm2 positive cells by immunofluorescence microscopy in pRb and CenpA co-depleted cells showed a mitotic index (left panel) close to that of wild-type cells. Graph showing (right panel) a large reduction in the percentage of hypodiploid mitosis (<2N) in pRb/CENP-A co-depleted cells (siRB/CenpA) in comparison with pRb depleted cells (siRB). B) Graph showing reduction of cells with micronuclei in pRb/CENP-A co-depleted cells (siRB/CenpA) in comparison with pRb alone depleted cells (siRB). C) a- Examples of near diploid (2N), hypodiploid (<2N) and hyperdiploid (>2N) metaphases in pRb/CenpA co-depleted cells; b- β-tubulin (green) immunostaining revealed the presence of normal bipolar spindles in pRb/CENP-A co-depleted cells. Chromosomes were counterstained with DAPI (blue). D) Time-lapse video-microscopy of mitotic pRb/CenpA double knockdown cells expressing the H2B-GFP construct showing that they progress normally into mitosis.