Figure 5
From: CXCR4 expression heterogeneity in neuroblastoma cells due to ligand-independent regulation
Quantitative Analysis of SDF-1 Secretion by Neuroblastoma Cells. Conditioned media from several neuroblastoma cell lines was collected at maximum confluency. Conditioned media was concentrated from 12 ml to 0.5 ml by filter centrifugation using membranes with 3000 molecular weight cut-offs. Quantitative Analysis of SDF-1 was performed using an enzyme linked immunoadsorbent assay (ELISA). Result represents the mean of three experiments for representatives from each surface expressing class compared to media background levels (*P < 0.005 and **P > 0.05, unpaired t-test).Concentrations are expressed in pg/ml.