Figure 5

Mn58b treatment slowed tumor growth through the inhibition of Akt(ser473) phosphorylation. A, Immunosuppressed mice were injected with MDA-MB 231 cells on each flank and tumors were allowed to grow to 0.1 cm³. At week 3.5, Mn58b or vehicle, were administrated to the mice intraperitoneally and the growth of tumor monitored. B, 11 xenografts excised from the mice treated with vehicle (C1-6) or Mn58b (T7-11) were frozen and lysed for western blot with the indicated antibodies or D, fixed in paraformaldehyde and section for immunohistochemistry staining with anti-total Akt and anti-pAkt(ser473). C, pAkt(ser473) signal was quantified with Image J program and normalized to total Akt. The ratios were input as scatter plot and analyzed using unpaired t-test with Prism (GraphPad). ** p < 0.05.