Figure 2

PP2 and dasatinib induced G ₁ cell cycle arrest, accompanied by apoptosis in B lymphoma cells. Cultures of 0.5 × 10⁶/ml human SudHL-4 cells (Panel A) or murine BKS-2 (B) were treated with 10 μM PP2 (SFK inhibitor), PP3 (inactive control analog), or 100 nM dasatinib (dual BCR-ABL and SFK inhibitor) for 48 hr. Then the cells were spun down and stained with PI for cell cycle analysis as described in the Materials and Methods section. (C) 0.5 × 10⁶/ml WEHI-231 cells were treated with 5 μM PP2 or an equivalent amount of DMSO for 48 hrs. Then the cells were spun down and stained with PI and AnnexinV-FITC for apoptosis analysis as described in the Materials and Methods section. (D) BKS-2 cells were treated with or without the indicated amount of PP1 (also a SFK inhibitor) or PP2 for 24 hrs. The cell lysates were probed for cyclin D2, then stripped and re-probed for β-actin. The ODs for cyclin D2 were normalized to the corresponding bands for β-actin and depicted as a fold change over no inhibitor.