EF24 inhibits hFANCD2-Ub and foci formation in HeLa cells and is more active than curcumin. A) Hydroxyurea-induced FANCD2-Ub in HeLa cells is inhibited by EF24 at lower concentrations than curcumin. hFANCD2-Ub was monitored by immunoblot and densitometry analysis to determine IC50. B) Hydroxyurea-induced hFANCD2 foci formation is inhibited by EF24. hFANCD2 foci were detected by immunofluorescence and the percentage of cells with more than 5 foci was determined for each treatment. Histograms represent the average of 3 experiments. Error bars represent standard deviation.