Figure 5

Real-Time TMA miRNA assays with integrated target capture. A) Capture and detection of known inputs of synthetic miR-221 added directly to STM buffer or to STM containing 10 ng of total cellular RNA (derived from VCaP or LNCaP cells, as indicated). Control reactions were run with synthetic miR-221 spiked directly into amplification reagent (without target capture). B) miR-221 copy levels determined by a commercial TaqMan RT-qPCR assay. C) miR-221 copy levels determined by our real-time TMA assay (with integrated target capture and amplification/detection). D) miR-221 copy numbers determined in prostate cancer xenografts using a commercial TaqMan RT-qPCR assay and also our research prototype real-time TMA assay (again incorporating target capture). For all real-time TMA samples, 10 ng total RNA was assayed per reaction in triplicate.