CRF induces the motility of MCF7 in a wound healing assay. MCF7 cells were stimulated with 10-8 M CRF or vehicle (control) and photographed at 0, 6, 12 and 24 hours after disruption of a small area of the cell layer. The remigration of the cells was quantified by measuring the distance at 3 at least different positions on each image with the program Image J, and expressed the distance as % of the average. Results represent the average of three independent experiments. *p < 0.05; **p < 0.01 compared to control cells at the same time point.