Figure 1

Schematic illustration of the HMGA2 gene. Locations of BAC clones RP11-299L9 (FITC) and RP11-427K2 (Cy3) and primers for the qRT-PCR reaction for exons 1–2 (F _{Ex 1–2} : forward primer and R _{Ex 1–2} : reverse primer) and exons 4–5 (F _{Ex 4-5} : forward primer and R _{Ex 4-5} : reverse primer) are indicated. The lower part shows an enlargement of the 3'UTR, where the base pair positions are based on the nucleotide sequence of NM_003483. Reported (Lee and Dutta, 2007) binding sites for let-7 are shown as dark boxes. Regions 1, 2 and 3 represent the sequences amplified by PCR; the locations of forward (F) and reverse (R) primers for each region are indicated. For details regarding primers, see Additional file 3.