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Table 1 RT-PCR and genomic PCR analyses of the 3' UTR of HMGA2

From: Expression levels of HMGA2 in adipocytic tumors correlate with morphologic and cytogenetic subgroups

   

RT-PCR products Region

Genomic PCR products Region

Case

Sample

Type of HMGA2 expression

1

2

3

1

2

3

 

Amniocytes 1

ND

+

+

+

ND

ND

ND

 

Amniocytes 2

ND

+

+

+

ND

ND

ND

 

Amniocytes 3

ND

+

+

+

ND

ND

ND

4

Lipoma t(3;12)

Differential

(+)

-

(+)

+

+

+

4

Peripheral blood

ND

ND

ND

ND

+

+

+

10

Lipoma t(5;12)

Aberrant

+

(+)

+

+

+

+

11

Lipoma der(12q13-15)

Differential

-

-

-

+

+

+

13

Lipoma der(12q13-15)

Aberrant

-

-

-

+

+

+

17

Variant Lipoma

Aberrant

(+)

-

(+)

+

+

+

18

Variant Lipoma

Aberrant

(+)

-

-

+

+

+

18

Peripheral blood

ND

ND

ND

ND

+

+

+

21

Lipoma der(6p21-22)

Differential

-

-

(+)

ND

ND

ND

26

Lipoma del(13q)

Differential

-

-

-

+

+

+

26

Peripheral blood

ND

ND

ND

ND

+

+

+

27

Lipoma del(13q)

Aberrant

-

-

-

ND

ND

ND

36

Angiolipoma

Aberrant

(+)

-

+

ND

ND

ND

37

Angiolipoma

Aberrant

-

-

(+)

+

+

+

38

Angiolipoma

Aberrant

-

-

(+)

+

+

+

38

Peripheral blood

ND

ND

ND

ND

+

+

+

39

Angiolipoma

Weak

-

-

-

+

+

+

39

Peripheral blood

ND

ND

ND

ND

+

+

+

40

Angiolipoma

Weak

-

-

-

+

+

+

40

Peripheral blood

ND

ND

ND

ND

+

+

+

41

Angiolipoma

ND

-

-

-

+

+

+

42

Angiolipoma

ND

-

-

(+)

+

+

+

43

Angiolipoma

ND

-

-

-

+

+

+

49

Hibernoma

Aberrant

-

-

(+)

+

+

+

  1. Abbreviations: Aberrant = Log10 value of ≥ 1 for exons 1–2 and exons 4–5; Differential = Aberrant expression of exons 1–2 with ≥ 10 times higher expression than for exons 4–5; Weak = Log10 value 0–1 for exons 1–2 and 4–5; + = strong PCR product; (+) = weak PCR product; - = no PCR product; ND = not determined.