Figure 2
From: The chromatin remodelling factor BRG1 is a novel binding partner of the tumor suppressor p16INK4a
BRG1 binds p16INK4a in melanoma cells and normal fibroblasts. A 50 μg of total cell lysates derived from uninduced (-) and induced (+) WMM1175_p16INK4a cells and WS-1 fibroblasts (passage 20) were separated using a 15% SDS-PAGE gel. Immunoblots were probed for p16INK4a and β-actin as indicated. B WMM1175_p16INK4a cells were induced to express p16INK4a with 4 mM IPTG or mock treated for 72 hours. Immunoprecipitations were performed using a mouse anti-p16INK4a antibody or a matched mouse IgG from nuclear cell lysate, as indicated. Immunoblots were probed for endogenous BRG1 and induced p16INK4a using a mouse anti-BRG1 and rabbit anti-p16INK4a, respectively. C Endogenous BRG1 was co-immunoprecipitated with p16INK4a from WS-1 normal dermal human fibroblasts grown to passage 20 as detailed above.