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Table 3 Total Metabolite and 13C-enriched metabolite content of paired non-cancerous and cancerous lung tissues from [U-13C]-glucose administered patientsA

From: Altered regulation of metabolic pathways in human lung cancer discerned by 13C stable isotope-resolved metabolomics (SIRM)

Compound

#6

sqCB, grade II

#7

adenoCB, grade II

#8

sqCB, grade II-III

#9

sqCB, grade II

#10

sqCB, grade III

 

NB

CB

N

C

N

C

N

C

N

C

Total Ala

4.67

15.76

6.25

6.44

4.97

9.28

5.14

15.08

5.42

27.34

13C-AlaC

0.03

0.28

0.15

0.34

0.09

0.57

0.21

0.79

0.29

1.60

Total Asp

1.32

4.27

3.83

3.09

2.67

1.51

1.94

4.78

2.27

1.66

13C-AspC

0.18

0.59

0.36

0.44

0.35

0.24

0.21

0.82

0.36

0.31

13C3-AspC, D

< 0.004

(< 0.3%)

0.058

(1.4%)

0.009

(0.2%)

0.005

(0.16%)

0.006

(0.2%)

0.009

(0.6%)

< 0.004

(< 0.2%)

0.024

(0.5%)

0.004

(0.17%)

0.020

(1.2%)

Total CitB

0.60

1.32

1.21

1.18

1.28

1.56

0.77

1.53

0.74

1.37

13C-CitB, C

0.03

0.10

0.02

0.05

0.08

0.12

0.03

0.11

< 0.004

0.12

Total Glu

0.24

4.80

2.96

2.97

1.88

3.25

1.91

4.78

1.82

3.28

13C-GluC

0.03

0.16

< 0.004

< 0.004

< 0.004

0.22

< 0.004

0.36

0.10

0.36

Total Gln

0.56

7.68

1.41

1.02

0.64

1.32

0.74

2.71

0.79

2.99

13C-GlnC

0.07

0.34

0.07

0.00

0.04

0.00

0.00

0.21

0.00

0.04

Total LacB

2.66

29.55

11.80

10.62

9.44

17.72

19.52

25.31

8.27

29.67

13C-LacC

< 0.004

0.66

0.19

0.63

0.19

0.88

< 0.004

1.44

0.03

0.94

Total SuccB

0.17

1.34

0.50

0.70

0.89

1.84

0.64

1.59

1.14

3.13

13C-SuccC

0.02

0.08

0.01

0.03

0.03

0.11

0.08

0.14

0.06

0.19

  1. A in μmole/g dry weight as determined by GC-MS; the %RSD was generally < 3% in triplicate analyses with the exception of Gln (11%) and 13C3-Asp (27%).
  2. B sqC: squamous cell carcinoma; adenoC: adenocarcinoma; N: non-cancerous; C: cancer; Cit: citrate; Lac: lactate; Succ: succinate
  3. C13C enrichment in excess of natural abundance; values in bold represent enhanced enrichment in cancer over its non-cancerous counterpart
  4. D13C mass isotopomer of Asp with three carbons labeled; detection limit was 0.004 μmole/g dry weight. Values in parentheses are the percentages of 13C3 isotopomers of the total aspartate. This was much greater than 5 × 10-4 % at natural abundance.
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Molecular Cancer

ISSN: 1476-4598