Figure 4
From: Inhibition of p70S6K2 down-regulates Hedgehog/GLI pathway in non-small cell lung cancer cell lines
Inhibition of p70S6K2 causes GLI1 degradation through reduction in phospho-GSK3β at Ser9 residue. A, Phosphorylated-GSK3β level of cells treated with p70S6K2 siRNA. Cell lysates were recovered and subjected to western blotting analysis to detect total p70S6K2, total GSK3β and phospho-GSK3β respectively at the indicated time points after siRNA transfection. B, GLI1 protein level of cells treated with p70S6K2-siRNA. A549 cells were also treated with siRNAs (control, p70S6K1, p70S6K2, and GLI1), and cell lysate was subjected to western blotting with GLI1, p70S6K1 and p70S6K2 antibodies 72 hr after transfection.