Effect of NFκB inhibition on TNFα mediated endothelial cell OPG production. HuDMECs were treated with the NFκB inhibitor PDTC (50 μM), TNFα (10 ng/ml) or TNFα (10 ng/ml) in conjunction with PDTC (50 μM) for 24 hours. RNA was extracted and gene expression quantified using real-time PCR (a). For real-time quantitative PCR, values were normalised to GAPDH and are given as fold expression compared to untreated HuDMECs. OPG secretion was measured in the conditioned medium using ELISA as described in materials and methods (b). Data are represented as mean ± S.E.M. from three independent experiments performed in triplicate. ***, p < 0.001.