Figure 2
From: Transcriptional activation of the Lats1 tumor suppressor gene in tumors of CUX1 transgenic mice
CDP/Cux p110 and p75 isoforms increases Lats1 transcription activity in reporter assays. (A) Diagram of the LATS1 reporter construct. The promoter region of the LATS1 gene, from nt -1463 to +310, was cloned into a luciferase reporter plasmid. (B) Hs578T cells were transfected with the LATS1 reporter plasmid together with vectors expressing either p200, p110 or p75 CUX1 or with an empty vector. 24 hours after transfection, whole cell extracts were prepared and processed to measure luciferase activity. The results are expressed as relative light units (RLU), normalized to β-galactosidase activity from an internal control and the standard deviation of 3 transfections is shown. (C) NIH3T3 cells were transfected with the LATS1 reporter plasmid together with increasing amount of a vector expressing p110 CUX1 or with an empty vector. Samples were processed as detailed in (B).