Figure 3
From: Transcriptional activation of the Lats1 tumor suppressor gene in tumors of CUX1 transgenic mice
Endogenous Lats1 expression is regulated by CUX1. RNA was prepared from mouse embryo fibroblasts and various cell lines expressing either p110 or p75 CUX1 or carrying an empty vector, as indicated. Lats1 mRNA expression was analyzed by RT-PCR using GAPDH as a control. Amplicons were resolved by agarose gel electrophoresis (A-D). (A) Mouse embryo fibroblasts (MEFs) derived from cux1-/- and cux1+/+ mice. (B) Lats1 mRNA expression was measured by quantitative real-time PCR in cux1-/- MEF and Hs578T human breast tumor cells expressing p110 CUX1 or carrying an empty vector. GAPDH levels were used to normalize the samples. The values are the mean of three measurements and error bars represent standard deviation. (C) MCF10A human mammary epithelial cells (D) NMuMG mouse mammary epithelial cells.