Figure 6
From: Transcriptional activation of the Lats1 tumor suppressor gene in tumors of CUX1 transgenic mice
Overexpression of Lats1 in NMuMG epithelial cells does not hinder cell cycle progression. (A) RNA was prepared from NMuMG mouse epithelial cells expressing p110 CUX1 or carrying an empty vector. Lats1 mRNA expression was analyzed by RT-PCR using GAPDH as a control. (B) Cells were synchronized at the G1/S transition using the double thymidine block procedure, and then released from the block and allowed to progress in the cell cycle. Nuclei were stained with propidium iodide and DNA content was determined by fluorescence-activated cell sorting (FACS) analysis. Cell cycle profiles were analyzed using the Watson model and the FlowJo™ software. The numbers below the FACS profiles indicate the percentage of cells with 2N DNA content (G0/G1 cells), between 2N and 4N DNA content (S phase cells) and 4N DNA content (G2/M cells).