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Figure 2 | Molecular Cancer

Figure 2

From: Vesnarinone downregulates CXCR4 expression via upregulation of Krüppel-like factor 2 in oral cancer cells

Figure 2

Downregulation of CXCR4 by vesnarinone. (a) CXCR4 mRNA was examined by quantitative PCR in B88 cells treated with chemotherapeutic agents (CDDP, 5-FU, or vesnarinone) or vehicle DMSO. (b) B88 cells treated with or without chemotherapeutic agents were incubated with or without anti-CXCR4 monoclonal antibody. Then the cells were incubated with PE-labeled goat anti-mouse IgG and analyzed by flow cytometry in order to determine the expression of CXCR4 protein. The white zones show the cells treated with vesnarinone stained by mouse IgG isotype control. The black zones show cells treated with vesnarinone, and gray zones show the cells treated with DMSO (upper), 5-FU (middle), or CDDP (lower), respectively. The black zones and gray zones were stained by anti-CXCR4 monoclonal antibody. (c) Vesnarinone downregulates CXCR4 mRNA and upregulates KLF2 mRNA in a time-dependent manner in ACC-M cells. ACC-M cells were treated with vesnarinone and quantitative RT-PCR was performed.

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Molecular Cancer

ISSN: 1476-4598