Figure 3
Inhibition of migration and lymph node metastasis by vesnarinone in B88 cells. (a) B88 cells were seeded on the upper membrane in the presence or absence of SDF-1α in the lower compartment, and in the presence of vesnarinone in the upper compartment. After 24 h, cells attached to the lower surface of the membrane were subjected to H&E staining and were counted. The bars show the SD of triplicate samples. Data are representative of three separate experiments with similar results. *, Not significant as determined by one-way ANOVA. (b, c, d and e) B88-luc cells (2 × 106) were inoculated into the masseter muscle of nude mice, which were treated with or without vesnarinone and were sacrificed at day 14 (d) or day 28 (b, c, d and e). Extirpated lymph nodes (b), H&E staining of the lymph nodes (c) (bar, 50 μm), and the weight of lymph nodes (d) are shown. *, Significant as determined by one-way ANOVA (vs. controls, P < 0.01). (e) Lymph nodes were lysed and then luciferase activity was measured. *, Significant as determined by one-way ANOVA (vs. controls, P < 0.01).